Reporter

Part:BBa_K313003:Design

Designed by: Ryosuke Kamei, Asahito Kaneko, Kosuke Uekusa, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-20)


terminator(reverse direction) and gfp (reverse direction)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209


Design Notes

We designed primers that hybridize both ends of gfp-terminator and have prefix and suffix in reverse direction.


Source

It was cloned from BBa_E0840 using PCR.

References