Reporter
Part:BBa_K313003:Design
Designed by: Ryosuke Kamei, Asahito Kaneko, Kosuke Uekusa, Ryo Kariyazono Group: iGEM10_UT-Tokyo (2010-10-20)
terminator(reverse direction) and gfp (reverse direction)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 209
Design Notes
We designed primers that hybridize both ends of gfp-terminator and have prefix and suffix in reverse direction.
Source
It was cloned from BBa_E0840 using PCR.